Castanea sativa Mill. Shells Aqueous Extract Exhibits Anticancer Properties Inducing Cytotoxic and Pro-apoptotic Effects[v1]

[ad_1]

Preprint
Write-up
Version 1
This version is not peer-reviewed

Version 1
: Received: 30 July 2019 / Authorized: 1 August 2019 / On the net: 1 August 2019 (04:37:49 CEST)

Cacciola, N.A. Squillaci, G. D’Apolito, M.. Petillo, O. Veraldi, F. La Cara, F. Peluso, G. Margarucci, S. Morana, A. Castanea sativa Mill. Shells Aqueous Extract Exhibits Anticancer Properties Inducing Cytotoxic and Pro-apoptotic Effects. Preprints 2019, 2019080006 (doi: 10.20944/preprints201908.0006.v1).

Cacciola, N.A. Squillaci, G. D’Apolito, M.. Petillo, O. Veraldi, F. La Cara, F. Peluso, G. Margarucci, S. Morana, A. Castanea sativa Mill. Shells Aqueous Extract Exhibits Anticancer Properties Inducing Cytotoxic and Pro-apoptotic Effects. Preprints 2019, 2019080006 (doi: 10.20944/preprints201908.0006.v1).

Abstract

In this study, bioactive compounds present in chestnut shells (CS) – derived from the industrial peeling processing – have been extracted by means of distinct procedures. The aqueous extracts have been characterized and the highest extraction yield and total phenolic content material was obtained by Standard Liquid Extraction (CLE). Gallic and protocatechuic acids have been the key straightforward phenols in the extract with 86.97 and 11.20 mg/g chestnut shells dry extract (CSDE), respectively. Six tumor cell lines (DU 145, Computer-three, LNCaP, MDA-MB-231, MCF-7 and Hep G2) and a single regular prostate epithelial cell line (PNT2) have been exposed to rising concentration of CSDE (1-100 µg/mL) for 24 h and cell viability was evaluated utilizing MTT assay. A lowered price in cell viability was observed in DU 145, Computer-three, LNCaP and MCF-7 cells even though viability of MDA-MB-231 and Hep G2 cells was not impacted except for PNT2 cells at a concentration of 100 µg/mL. Moreover, CSDE-at concentrations of 55.five and 100 µg/mL- lead to a substantial raise of apoptotic cells in DU 145 cells of 28.two% and 61%, respectively. In conclusion, our findings showed that CS could possibly be employed for the extraction of numerous polyphenols that might represent superior candidates for option therapies or in mixture with present chemotherapeutics.

Topic Places

chestnut shells polyphenols bioactive compounds apoptosis cytotoxicity human cell lines

This is an open access short article distributed below the Inventive Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, offered the original function is effectively cited.

Comments ()

We encourage comments and feedback from a broad variety of readers. See criteria for comments and our diversity statement.

[ad_2]

Latest posts