Cannabis sativa is a medicinal herb with a diverse variety of chemotypes that can exert each anxiolytic and anxiogenic effects on humans. Health-related cannabis patients getting organically grown cannabis from a single supply had been surveyed about the effectiveness of cannabis for treating anxiousness. Sufferers rated cannabis as very powerful general for treating anxiousness with an typical score of eight.03 on a Likert scale of to 10 ( = not powerful, 10 = particularly powerful). Sufferers also identified which strains they identified the most or least powerful for relieving their symptoms of anxiousness. To obtain correlations amongst anxiolytic activity and chemotype, the best 4 strains voted most and least powerful had been analyzed by HPLC-MS/MS to quantify cannabinoids and GC-MS to quantify terpenes. Tetrahydrocannabinol (THC) and trans-nerolidol have statistically important correlations with elevated anxiolytic activity. Guiaol, eucalyptol, γ-terpinene, α-phellandrene, three-carene, and sabinene hydrate all have important correlations with decreased anxiolytic activity. Additional research are necessary to much better elucidate the entourage effects that contribute to the anxiolytic properties of cannabis varieties.