Metastasis is the principal trigger of death in sufferers with melanoma. Cannabis-primarily based medicines are helpful adjunctive drugs in cancer patients. Tau and Stathmin proteins are the important proteins in cancer metastasis. Right here we have investigated the impact of a standardized Cannabis sativa extract on cell migration and Tau and Stathmin gene expression in the melanoma cell line.
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In the therapy group, melanoma (B1617) was treated 48 hr with a variety of concentrations of standardized C. sativa extract. Cells with no therapy were regarded as the manage group, then study was followed by Quantitative RT-True Time PCR assay. Relative gene expression was calculated by the ΔΔct process. Migration assay was utilized to evaluate cancer metastasis.
Tau and stathmin gene expression was drastically decreased compared to the manage group. Cell migration was also drastically decreased compared to controls.
C. sativa decreased tau and stathmin gene expression and cancer metastasis. The final results might have some clinical relevance for the use of cannabis-primarily based medicines in sufferers with metastatic melanoma.
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Vaseghi G1, Taki MJtwo, Javanmard SHtwo.